In this study, apoptosis and autophagy of PC-3 cells in human prostate cancer were observed after treatment with T2A and its relevance. The interaction between autophagy and apoptosis of Tanshinon IIA in prostate cancer cells. The interaction between autophagy and Tanshinon IIA apoptosis in prostate cancer cells. All these results demonstrated that autophagy is the cytoprotection mechanism of this experimental system and that RSOs as a result of T2A treatment played a crucial role in early apoptosis and autophagy. In addition, the z-VAD-fmk apoptosis suppressor could not facilitate the formation of autophagic vacuoles, which also demonstrated that T2A-induced autophagy occurs independently of apoptosis.