Therefore, the results of our current study demonstrate the potential benefits of Med-AP in treating cancer as an apoptosis inductor and a potential target for actin. This finding largely supports the effect of Honeys AF in inhibiting progressive malignant activity by disrupting the interaction between the actin cytoskeleton and the EFGR signaling system. Therefore, gene and protein expression levels indicating cytochrome C activation and the release of caspase 9 eventually led to the release of caspase 3 in honey AF. Melitin from Apis poison flowers as a promising therapy for skin cancer treatment. Honey-AF manipulated the location of actin by capturing the tension fibers located under the membrane, resulting in defective cytoskeletal organization of actin. Toxin Melitin as a promising therapeutic agent for the treatment of skin cancer In this study, Med-AF showed significant antiproliferative potential for A375 cells. Subsequently, the cellular mechanism of Mel-AP, which causes cell death due to apoptosis, was studied. It is recommended to seek advice from a licensed health care professional before conducting natural, integrative or routine treatment. Providing the information contained in this document does not diagnose, treat, facilitate or prevent any disease or illness. Melitin, an important component of bee venom, is produced by bees. Therefore, the term EGFR, which binds protein to F-actin, was also excluded. Subsequently, it was found that cell death at apoptosis occurs along the internal mitochondrial pathway.