Chiconin causes cell death by inhibiting glycolysis of I-10 cells from human testicular cancer and TSAM-2 cells from seminoma. OBJECTIVE: To study the death patterns of chiconin-induced cells in human I-10 cell cancer and TCAM-2 seminoma and to investigate a possible mechanism in light of mitochondrial function and glycolysis. Chiconin causes cell death by inhibiting glycolysis in human I-10 cancerous cells and TCAM-2 cells in seminoma. CONCLUSIONS: Xyconin can inhibit proliferation, induce apoptosis and increase autophagy in I-10 and TCAM-2 cells, potentially affecting cellular energy metabolism. Western staining was used to determine relative levels of expression of cut-off apoptotic proteins Bax, Bcl-2 and caspase 3, autophagous protein LC3B and glycolysis related proteins PKM2, GLUT1 and HK2. The inhibitory effect of chiconin on cell proliferation was evaluated by an MTT test. The intracellular content of lactic acid in cells was determined using a lactic acid kit. The information contained in this document does not constitute a diagnosis, treatment, mitigation or prevention of any disease or medical condition. It is recommended that you seek advice from a licensed physician before beginning any natural, integrative or routine treatment. Over 500 pages of alternatives and information about natural medicine. This site is for information purposes only. If not, please click here to become a member.